LOCALIZATION OF A PRIMATE-SPECIFIC ESTERASE USING IMMUNOFLUORESCENCE AND IMMUNOPEROXIDASE TECHNIQUES
نویسندگان
چکیده
منابع مشابه
Comparison of techniques and immunoreagents used for indirect immunofluorescence and immunoperoxidase identification of enteroviruses.
Peroxidase-conjugated antibodies were found to be as sensitive as those conjugated to fluorescein-isothiocyanate (FITC) for identification of selected enterovirus types by the indirect technique. Peroxidase conjugates, however, were found to give fewer nonspecific reactions. The reasons for the higher specificity of the immunoperoxidase technique appear to be related to the relative size, charg...
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We describe a method for the simultaneous identification of up to three Mycoplasma species by the use of contrast-labeled fluorescent antibodies against two species and peroxidase-labeled antibody against a third species. The procedure enabled the rapid identification of colonies of three artificially mixed avian Mycoplasma species on agar blocks and also mixtures of species in cultures from na...
متن کاملIntracellular Distribution of a Primate-specific Esterase in Cultured Cells and Tissues
GEORGIRENE D. VLADUTIU and NOEL R. ROSE. From the Center for Immunology and the Department of Microbiology, School of Medicine, State University of New York at Buffalo, Buffalo, New York 14214 . Dr . Vladutiu's present address is the Department of Pediatrics, Division of Human Genetics, Children's Hospital of Buffalo, 86 Hodge Street, Buffalo, New York 14222 . Dr . Rose's present address is the...
متن کاملApplication of Specific Monoclonal Antibodies for Characterization of Leishmania spp. Promastigotes Using Indirect Immunofluorescent and Immunoperoxidase Tests
Background: Different methods have been used for characterization of Leishmania promastigotes. Monoclonal antibodies are useful in characterization of Leishmania spp . both amastogotes and promastigotes. Objective: Comparing the characterization of Leishmania spp. promastigotes with immunoperoxidase test (Avidin-Biotin) techniques and an indirect immunofluorescent assay (IFA). Methods: Ap...
متن کاملPlasma cell counts on human jejunal biopsy specimens examined by immunofluorescence and immunoperoxidase techniques: a comparative study.
Ten human jejunal biopsy specimens were examined by both immunofluorescence (IF) and immunoperoxidase (IP) methods to compare both plasma cell counts and the distribution of extracellular immunoglobulins. Each specimen was cut into at least two portions, one fixed in 5% formaldehyde in phosphate-buffered saline before being snap-frozen and sectioned on a cryostat for IF, the other being fixed i...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1973
ISSN: 0022-1554,1551-5044
DOI: 10.1177/21.6.559